complete mesencult tm osteogenic medium Search Results


complete mouse mesencult osteogenic medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc complete mouse mesencult osteogenic medium
SSCs generate PDPN-expressing progenies with a phenotype identical to that of epiphyseal PDPN-expressing stromal cells. A , representative flow cytometric data of in vitro SSC progenies generated from primary isolated SSCs during culture with <t>MesenCult.</t> B , PDPN and Sca-1 expression in in vitro generated SSC progenies. C – E , representative ICC images of the in vitro PDPN-expressing SSC progenies stained with PDPN/PDGFRβ/DAPI ( C ), PDPN/NG2/DAPI ( D ), and PDPN/αSMA/DAPI ( E ). Scale bars indicate 50 μm. αSMA, α-smooth muscle actin; DAPI, 4′,6-diamidino-2-phenylindole; ICC, immunocytochemistry; NG2, neuron-glial antigen-2; PDGFRβ, platelet-derived growth factor receptor-β; Sca-1, stem cell antigen-1; PDPN, podoplanin; SSC, skeletal stem cell.
Complete Mouse Mesencult Osteogenic Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
complete mouse mesencult osteogenic medium - by Bioz Stars, 2026-08
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SSCs generate PDPN-expressing progenies with a phenotype identical to that of epiphyseal PDPN-expressing stromal cells. A , representative flow cytometric data of in vitro SSC progenies generated from primary isolated SSCs during culture with MesenCult. B , PDPN and Sca-1 expression in in vitro generated SSC progenies. C – E , representative ICC images of the in vitro PDPN-expressing SSC progenies stained with PDPN/PDGFRβ/DAPI ( C ), PDPN/NG2/DAPI ( D ), and PDPN/αSMA/DAPI ( E ). Scale bars indicate 50 μm. αSMA, α-smooth muscle actin; DAPI, 4′,6-diamidino-2-phenylindole; ICC, immunocytochemistry; NG2, neuron-glial antigen-2; PDGFRβ, platelet-derived growth factor receptor-β; Sca-1, stem cell antigen-1; PDPN, podoplanin; SSC, skeletal stem cell.

Journal: The Journal of Biological Chemistry

Article Title: Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development

doi: 10.1016/j.jbc.2022.101833

Figure Lengend Snippet: SSCs generate PDPN-expressing progenies with a phenotype identical to that of epiphyseal PDPN-expressing stromal cells. A , representative flow cytometric data of in vitro SSC progenies generated from primary isolated SSCs during culture with MesenCult. B , PDPN and Sca-1 expression in in vitro generated SSC progenies. C – E , representative ICC images of the in vitro PDPN-expressing SSC progenies stained with PDPN/PDGFRβ/DAPI ( C ), PDPN/NG2/DAPI ( D ), and PDPN/αSMA/DAPI ( E ). Scale bars indicate 50 μm. αSMA, α-smooth muscle actin; DAPI, 4′,6-diamidino-2-phenylindole; ICC, immunocytochemistry; NG2, neuron-glial antigen-2; PDGFRβ, platelet-derived growth factor receptor-β; Sca-1, stem cell antigen-1; PDPN, podoplanin; SSC, skeletal stem cell.

Article Snippet: After 24 h, the culture medium was replaced with the Complete mouse MesenCult Osteogenic Medium (Stem Cell Technologies) containing L-glutamine (Wako) and penicillin/streptomycin/amphotericin B (Wako), and the cells were cultured for 12 days.

Techniques: Expressing, In Vitro, Generated, Isolation, Staining, Immunocytochemistry, Derivative Assay